coding sequences (Twist Bioscience)
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Coding Sequences, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/coding+sequence/coding+sequences+synthetic/bio_rxiv__64898__2026__05__17__725801-378-1-30
Average 86 stars, based on 1 article reviews
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Sequencing:Article Title: Metabolic engineering of stomatal precursor cells enhances photosynthetic water‐use efficiency and vegetative growth under water‐deficit conditions in Arabidopsis thaliana Article Snippet: .. Appropriate flanking regions compatible with Golden Gate Assembly (Marillonnet and Engler, ) were added to the 5' and 3' regions, and the resulting Article Title: Systematic investigation of Epstein-Barr virus transcriptional regulator interactions with the human genome Article Snippet: The pcDNA5/FRT/TO backbone and DNA sequences for EBV vTRs from strain B95-8 were supplied to Twist Bioscience to generate untagged, N-terminally GFP tagged and C-terminally GFP tagged plasmids. .. The Article Title: Advancing Vibrio genetics: A platform for efficient genomic manipulation Article Snippet: To add chloramphenicol resistance, the selectable marker used during genomic integration and counter-selection, a DNA fragment was synthesized consisting of the cat sequence from pBACe3.6 with a single FRT site upstream of the coding sequence (Twist Biosciences)( ). .. To add chloramphenicol resistance, the selectable marker used during genomic integration and counter-selection, a DNA fragment was synthesized consisting of the cat sequence from pBACe3.6 with a single FRT site upstream of the Article Title: Site-specific deacylation by ABHD17a controls BK channel splice variant activity Article Snippet: .. A catalytically inactive version of ABHD17a was made by mutating amino acids Ser 170 and Asp 255 in the catalytic triad to alanine by synthesizing the entire Article Title: A CRISPRi Gene Regulation System for Bifidobacteria Article Snippet: A 500 bp fragment of the integrated erythromycin resistance marker present in B. breve dCas9 strains was amplified by PCR and cloned into pFREM29‐CAT using NotI and XbaI (New England Biolabs) to generate pFREM29‐CAT‐E500. .. The Article Title: Development of a Biosafety Level 1 Cellular Assay for Identifying Small-Molecule Antivirals Targeting the Main Protease of SARS-CoV-2: Evaluation of Cellular Activity of GC376, Boceprevir, Carmofur, Ebselen, and Selenoneine Article Snippet: .. For Mpro expression, the Article Title: Chromosome-level assembly of basil genome unveils the genetic variation driving Genovese and Thai aroma types. Article Snippet: Jing Zhang, Mohamad Abu-Abied, Renana Milavski, Chen Adler, Alona Shachter, Tali Kahane-Achinoam, Hadas Melnik-Ben-Gera, Rachel Davidovich-Rikanati, Adrian F. Powell , David Chaimovitsh, Gon Carmi, Nativ Dudai, Susan R. Strickler and Itay Gonda Boyce Thompson Institute, Ithaca, New York, USA, Unit of Aromatic and Medicinal Plants, Newe Ya’ar Research Center, Volcani Institute, Ramat-Yishay, Israel, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel, Bioinformatics Unit, Newe Ya’ar Research Center, Agricultural Research Organization, Volcani Institute, Ramat-Yishay, Israel, Chicago Botanic Garden, Plant Science and Conservation, 1000 Lake Cook Rd., Glencoe, Illinois 60022, USA, and Plant Biology and Conservation Program, Northwestern University, 2145 Sheridan Rd., Evanston, Illinois 60208, USA Synthesized:Article Title: Metabolic engineering of stomatal precursor cells enhances photosynthetic water‐use efficiency and vegetative growth under water‐deficit conditions in Arabidopsis thaliana Article Snippet: .. Appropriate flanking regions compatible with Golden Gate Assembly (Marillonnet and Engler, ) were added to the 5' and 3' regions, and the resulting Article Title: Advancing Vibrio genetics: A platform for efficient genomic manipulation Article Snippet: To add chloramphenicol resistance, the selectable marker used during genomic integration and counter-selection, a DNA fragment was synthesized consisting of the cat sequence from pBACe3.6 with a single FRT site upstream of the coding sequence (Twist Biosciences)( ). .. To add chloramphenicol resistance, the selectable marker used during genomic integration and counter-selection, a DNA fragment was synthesized consisting of the cat sequence from pBACe3.6 with a single FRT site upstream of the Article Title: A CRISPRi Gene Regulation System for Bifidobacteria Article Snippet: A 500 bp fragment of the integrated erythromycin resistance marker present in B. breve dCas9 strains was amplified by PCR and cloned into pFREM29‐CAT using NotI and XbaI (New England Biolabs) to generate pFREM29‐CAT‐E500. .. The Article Title: Development of a Biosafety Level 1 Cellular Assay for Identifying Small-Molecule Antivirals Targeting the Main Protease of SARS-CoV-2: Evaluation of Cellular Activity of GC376, Boceprevir, Carmofur, Ebselen, and Selenoneine Article Snippet: .. For Mpro expression, the Article Title: Chromosome-level assembly of basil genome unveils the genetic variation driving Genovese and Thai aroma types. Article Snippet: Jing Zhang, Mohamad Abu-Abied, Renana Milavski, Chen Adler, Alona Shachter, Tali Kahane-Achinoam, Hadas Melnik-Ben-Gera, Rachel Davidovich-Rikanati, Adrian F. Powell , David Chaimovitsh, Gon Carmi, Nativ Dudai, Susan R. Strickler and Itay Gonda Boyce Thompson Institute, Ithaca, New York, USA, Unit of Aromatic and Medicinal Plants, Newe Ya’ar Research Center, Volcani Institute, Ramat-Yishay, Israel, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel, Bioinformatics Unit, Newe Ya’ar Research Center, Agricultural Research Organization, Volcani Institute, Ramat-Yishay, Israel, Chicago Botanic Garden, Plant Science and Conservation, 1000 Lake Cook Rd., Glencoe, Illinois 60022, USA, and Plant Biology and Conservation Program, Northwestern University, 2145 Sheridan Rd., Evanston, Illinois 60208, USA Marker:Article Title: Advancing Vibrio genetics: A platform for efficient genomic manipulation Article Snippet: To add chloramphenicol resistance, the selectable marker used during genomic integration and counter-selection, a DNA fragment was synthesized consisting of the cat sequence from pBACe3.6 with a single FRT site upstream of the coding sequence (Twist Biosciences)( ). .. To add chloramphenicol resistance, the selectable marker used during genomic integration and counter-selection, a DNA fragment was synthesized consisting of the cat sequence from pBACe3.6 with a single FRT site upstream of the FLAG-tag:Article Title: Site-specific deacylation by ABHD17a controls BK channel splice variant activity Article Snippet: .. A catalytically inactive version of ABHD17a was made by mutating amino acids Ser 170 and Asp 255 in the catalytic triad to alanine by synthesizing the entire Expressing:Article Title: A CRISPRi Gene Regulation System for Bifidobacteria Article Snippet: A 500 bp fragment of the integrated erythromycin resistance marker present in B. breve dCas9 strains was amplified by PCR and cloned into pFREM29‐CAT using NotI and XbaI (New England Biolabs) to generate pFREM29‐CAT‐E500. .. The Article Title: Development of a Biosafety Level 1 Cellular Assay for Identifying Small-Molecule Antivirals Targeting the Main Protease of SARS-CoV-2: Evaluation of Cellular Activity of GC376, Boceprevir, Carmofur, Ebselen, and Selenoneine Article Snippet: .. For Mpro expression, the Article Title: Chromosome-level assembly of basil genome unveils the genetic variation driving Genovese and Thai aroma types. Article Snippet: Jing Zhang, Mohamad Abu-Abied, Renana Milavski, Chen Adler, Alona Shachter, Tali Kahane-Achinoam, Hadas Melnik-Ben-Gera, Rachel Davidovich-Rikanati, Adrian F. Powell , David Chaimovitsh, Gon Carmi, Nativ Dudai, Susan R. Strickler and Itay Gonda Boyce Thompson Institute, Ithaca, New York, USA, Unit of Aromatic and Medicinal Plants, Newe Ya’ar Research Center, Volcani Institute, Ramat-Yishay, Israel, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel, Bioinformatics Unit, Newe Ya’ar Research Center, Agricultural Research Organization, Volcani Institute, Ramat-Yishay, Israel, Chicago Botanic Garden, Plant Science and Conservation, 1000 Lake Cook Rd., Glencoe, Illinois 60022, USA, and Plant Biology and Conservation Program, Northwestern University, 2145 Sheridan Rd., Evanston, Illinois 60208, USA Modification:Article Title: A CRISPRi Gene Regulation System for Bifidobacteria Article Snippet: A 500 bp fragment of the integrated erythromycin resistance marker present in B. breve dCas9 strains was amplified by PCR and cloned into pFREM29‐CAT using NotI and XbaI (New England Biolabs) to generate pFREM29‐CAT‐E500. .. The Clone Assay:Article Title: A CRISPRi Gene Regulation System for Bifidobacteria Article Snippet: A 500 bp fragment of the integrated erythromycin resistance marker present in B. breve dCas9 strains was amplified by PCR and cloned into pFREM29‐CAT using NotI and XbaI (New England Biolabs) to generate pFREM29‐CAT‐E500. .. The Article Title: Development of a Biosafety Level 1 Cellular Assay for Identifying Small-Molecule Antivirals Targeting the Main Protease of SARS-CoV-2: Evaluation of Cellular Activity of GC376, Boceprevir, Carmofur, Ebselen, and Selenoneine Article Snippet: .. For Mpro expression, the Article Title: Chromosome-level assembly of basil genome unveils the genetic variation driving Genovese and Thai aroma types. Article Snippet: Jing Zhang, Mohamad Abu-Abied, Renana Milavski, Chen Adler, Alona Shachter, Tali Kahane-Achinoam, Hadas Melnik-Ben-Gera, Rachel Davidovich-Rikanati, Adrian F. Powell , David Chaimovitsh, Gon Carmi, Nativ Dudai, Susan R. Strickler and Itay Gonda Boyce Thompson Institute, Ithaca, New York, USA, Unit of Aromatic and Medicinal Plants, Newe Ya’ar Research Center, Volcani Institute, Ramat-Yishay, Israel, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel, Bioinformatics Unit, Newe Ya’ar Research Center, Agricultural Research Organization, Volcani Institute, Ramat-Yishay, Israel, Chicago Botanic Garden, Plant Science and Conservation, 1000 Lake Cook Rd., Glencoe, Illinois 60022, USA, and Plant Biology and Conservation Program, Northwestern University, 2145 Sheridan Rd., Evanston, Illinois 60208, USA Functional Assay:Article Title: Chromosome-level assembly of basil genome unveils the genetic variation driving Genovese and Thai aroma types. Article Snippet: Jing Zhang, Mohamad Abu-Abied, Renana Milavski, Chen Adler, Alona Shachter, Tali Kahane-Achinoam, Hadas Melnik-Ben-Gera, Rachel Davidovich-Rikanati, Adrian F. Powell , David Chaimovitsh, Gon Carmi, Nativ Dudai, Susan R. Strickler and Itay Gonda Boyce Thompson Institute, Ithaca, New York, USA, Unit of Aromatic and Medicinal Plants, Newe Ya’ar Research Center, Volcani Institute, Ramat-Yishay, Israel, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel, Bioinformatics Unit, Newe Ya’ar Research Center, Agricultural Research Organization, Volcani Institute, Ramat-Yishay, Israel, Chicago Botanic Garden, Plant Science and Conservation, 1000 Lake Cook Rd., Glencoe, Illinois 60022, USA, and Plant Biology and Conservation Program, Northwestern University, 2145 Sheridan Rd., Evanston, Illinois 60208, USA Activity Assay:Article Title: Chromosome-level assembly of basil genome unveils the genetic variation driving Genovese and Thai aroma types. Article Snippet: Jing Zhang, Mohamad Abu-Abied, Renana Milavski, Chen Adler, Alona Shachter, Tali Kahane-Achinoam, Hadas Melnik-Ben-Gera, Rachel Davidovich-Rikanati, Adrian F. Powell , David Chaimovitsh, Gon Carmi, Nativ Dudai, Susan R. Strickler and Itay Gonda Boyce Thompson Institute, Ithaca, New York, USA, Unit of Aromatic and Medicinal Plants, Newe Ya’ar Research Center, Volcani Institute, Ramat-Yishay, Israel, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel, Bioinformatics Unit, Newe Ya’ar Research Center, Agricultural Research Organization, Volcani Institute, Ramat-Yishay, Israel, Chicago Botanic Garden, Plant Science and Conservation, 1000 Lake Cook Rd., Glencoe, Illinois 60022, USA, and Plant Biology and Conservation Program, Northwestern University, 2145 Sheridan Rd., Evanston, Illinois 60208, USA |
